Levente L. Diosady* and Taya Puzanov Department of Chemical Engineering and Applied Chemistry, University of Toronto, 200 College St., Toronto, Ontario, Canada M5S 3E5
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In the continuous membrane fermentation of Lactobacillus rhamnosus at biomass concentrations above 100 g/L the viscosity of the system increased sharply resulting in decreased permeate flux. At cell densities higher than 108 g/L a slight deviation from Newtonian behaviour was observed. Steady state was achieved and main kinetic characteristics remained constant for around 100 hours when the biomass concentration was maintained at a constant level by continuous bleeding.ABSTRACT
Keywords:
biomass bleeding; membrane fermentation; Lactobacillus rhamnosus; viscosity; rheology.
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[1] Aeschlimann, A. and Stockar, U. 1991. Continuous production of lactic acid from whey permeate by Lactobacillus helveticus in a cell-recycle reactor. Enzyme and Microbial Technology, 13: 811 –816.REFERENCES
[2] Bhugaloo-Vial, P., Grajek, W., Dousset, X., and Boyaval, P. 1997. Continuous bacteriocin production with high cell density bioreactors. Enzyme and Microbial Technology, 21: 450 –457.
[3] Cheryan, M. 1986. “Ultrafiltration Handbook”. Technomic Publishing Co. Inc., Lancaster, Pennsylvania.
[4] Crespo, J. P. S. G., Xavier, A. M. R. B., Barreto, M. T. O., Goncalves, L. M. D., Almeida, J. S., and Carrondo, M. J. T. 1992. Tangential flow filtration for continuous cell recycle culture of acidogenic bacteria. Chemical Engineering Science, 47: 205 –214.
[5] Goncalves, L. M. D., Xavier, A. M. R. B., Almeida, J. S., and Carrondo, M. J. T. 1991. Concomitant substrate and product inhibition kinetics in lactic acid production. Enzyme and Microbial Technology, 13: 314 –319.
[6] Jeantet, R., Maubois, J. L., and Boyaval, P. 1996. Semicontinuous production of lactic acid in a bioreactor coupled with nanofiltration membranes. Enzyme and Microbial Technology, 19: 615 –619.
[7] Major, N. C. and Bull, A. T. 1989. The physiology of lactate production by Lactobacillus delbrueckii in a chemostat with cell recycle. Biotechnology and Bioengineering, 34: 592 –599.
[8] Mehaia, M. A. and Cheryan, M. 1986. Lactic acid from acid whey permeate in a membrane recycle bioreactor. Enzyme and Microbial Technology, 8: 289 –292.
[9] Nagata, N., Herouvis, K.J., Dziewulski, D. M., and Belfort, G. 1989. Cross-flow membrane microfiltration of a bacterial fermentation broth. Biotechnology and Bioengineering, 34: 447 –466.
[10] Puzanov, T. 1999. Continuous production of lactic acid in a membrane bioreactor. M. A. Sc Thesis. Toronto, Canada: University of Toronto.
[11] Sumner, J. B. 1925. Journal of Bacteriology chemical, 65: 385-393.
[12] Vick Roy, T. B., Mandel, D. K., Dea, D. K., Blanch, H. W., and Wilke, C. R. 1983. The application of cell recycle to continuous fermentative lactic acid production. Biotechnology Letters, 5, 10: 665 – 670.
[13] Wicken, A. J., Ayres, A., Campbell, L. K., and Knox, K. W. 1983. Effect of growth conditions on production of rhamnose-containing cell wall and capsular polysaccharides by strains of Lactobacillus casei subsp. rhamnosus. Journal of Bacteriology, 153: 84 –92.
[14] Xavier, A. M. R. B., Goncalves, L. M. D., Moreira, J. L., and Carrondo, M. J. T. 1995. Operational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor. Biotechnology and Bioengineering, 45: 320 –327.
[15] Ye, K., Jin, S., and Shimizu, K. 1996. Cell recycle and broth reuse fermentation with cross-flow filtration and ion-exchange resin. Journal of Chemical technology and biotechnology, 24: 223 – 226.
[16] Zhang, D. and Cheryan, M. 1992. Starch to lactic acid in a continuous membrane bioreactor. Process Biochemistry, 29: 145 –150.
ARTICLE INFORMATION
Accepted:
2005-03-01
Available Online:
0205-04-03
Diosady, L.-L., Puzanov, T. 2005. Membrane fermentation of lactic acid. International Journal of Applied Science and Engineering, 3, 19–25. https://doi.org/10.6703/IJASE.2005.3(1).19
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