a Department of Pharmaceutical Chemistry, K. B. Institute of Pharmaceutical Education and Research, Sector-23, GH-6, Gandhinagar-382023, Gujarat, India. b Department of Phytochemistry and Pharmacognosy, L. J. Institute of Pharmacy, Near Nagdev Kalyan Mandir, Sanand Cross Roads, Ahmedabad, PIN Code 382 210 Gujarat, India.
A reproducible and reliable HPTLC method for the indirect determination of gymnemic acids as gymnemagenin in Gymnema sylvestre plant has been reported. As gymnemagenin, a common aglycone of gymnemic acids, lacks in conjugation, very poor UV absorption is observed. So post-derivatzation method was used for quantification of gymnemagenin. Linearity was observed in the range of 180 – 1440 ng/spot. Thus the method was found to be more sensitive where gymnemagenin was quantified at nanogram level. The method was validated as per ICH guidelines and successfully applied for quantification of gymnemagenin from plant leaf powder, extract and poly herbal marketed formulation. Percentage recovery was found to be 98.4 ± 1.0%.
Keywords: High Performance Thin-Layer Chromatography; Gymnemagenin, Standardizatio; Gymnema sylvestre.
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ARTICLE INFORMATION
Accepted: 2008-04-24 Available Online: 2008-04-01
Cite this article:
Trivedi, P.D., Pundarikakshudu, K. 2008. A validated high performance Thin-layer chromatographic method for the estimation of gymnemic acids through gymnemagenin in gymnema sylvestre, materials, extracts and formulations. International Journal of Applied Science and Engineering, 6, 19–28.https://doi.org/10.6703/IJASE.2008.6(1).19
