Alluri V. Krishnarajua, Tayi V. N. Raoa, Dodda Sundararajua, Mulabagal Vanisreeb, Hsin-Sheng Tsayb, and Gottumukkala V. Subbarajua*

Laila Impex Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada –520 007, Andhra Pradesh, India
Institute of Biotechnology, Chaoyang University of Technology, Wufong, Taichung 413, Taiwan


 

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ABSTRACT


Medicinal plants constitute an important component of flora and are widely distributed in India. The pharmacological evaluation of substances from plants is an established method for the identification of lead compounds which can leads to the development of novel and safe medicinal agents. Based on the ethnopharmacological literature, several species of medicinal plants used in traditional medicine in India were collected. In the present study, aqueous extracts of these medicinal plants were screened for their cytotoxicity using brine shrimp lethality test. Out of the 120 plants tested, Pistacia lentiscus exhibited potent brine shrimp lethality with LC50 2.5 μg. Aristolochia indica (Aristolochiaceae), Boswellia serrata (Burseraceae), Ginkgo biloba (Ginkgoaceae), Garcinia cambogia (Clusiaceae), and Semecarpus anacardium (Anacardiaceae) have also showed significant cytotoxicity with LC50 13, 18, 21, 22, and 29.5 μg respectively. The present study supports that brine shrimp bioassay is simple reliable and convenient method for assessment of bioactivity of medicinal plants and lends support for their use in traditional medicine.


Keywords: Artemia salina; brine shrimp lethality test; medicinal plants; cytotoxicity.


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ARTICLE INFORMATION




Accepted: 2005-05-26
Publication Date: 2005-10-03


Cite this article:

Krishnaraju, A.-V., Rao, Tayi V.-N. , Sundararaju, D., Vanisree, M., Tsay, H.-S., Subbaraju, G.-V., 2005. Assessment of bioactivity of Indian medicinal plants using brine shrimp (Artemia salina) lethality assay. International Journal of Applied Science and Engineering, 3, 125–134. https://doi.org/10.6703/IJASE.2005.3(2).125