T. Chandrasekhar, T. Mohammad Hussain*, G. Rama Gopal, and J. V. Srinivasa Rao 

Division of Plant Biotechnology, Department of Botany, Sri Venkateswara University, Tirupati-517 502, A.P, India

 

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ABSTRACT

An efficient procedure has been developed for inducing somatic embryogenesis from mature leaves of Tylophora indica (Burm.f.) Merrill, an important medicinal plant. Leaf sections were initially cultured on Murashige and Skoog's (MS) medium supplemented with thidiazuron (TDZ) in addition with 2, 4-dichlorophenoxy acetic acid (2,4-D), particularly 0.5 µm TDZ along with 1.5 µm 2,4-D was very effective in inducing somatic embryos. Plants were regenerated from in vitro somatic embryos plated on semisolid medium devoid of growth regulators. Plantlets were obtained in 65% of the cultures with 2% Sodium alginate coated embryos and control embryos showed 90 % germination. The percent of survival during hardening was 65 to 70. Regenerated plantlets continued to grow after transfer to a greenhouse environment and were similar phenotypically to zygotic seedlings. This simple regeneration system through somatic embryogenesis may be beneficial for mass propagation of Tylophora indica.


Keywords: Tylophora indica; somatic embryogenesis; thidiazuron.


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REFERENCES

[1] Shivpuri, D. N., Menon, M. P., and Prakash, D. 1968. Preliminary studies in Tylophora indica in the treatment of asthma and allergic rhinitis. Journal of Associaiton of Physicians India, 16 (1): 9-15.

[2] Anonymous, 1976. The Wealth of India. Raw Materials, Vol. X: Publications and Information Directorate. CSIR, New Delhi, India.

[3] Rao, K.V., Wilson, R. A., and Cummings, B. 1971. Alkaloids of tylophora. 3. New alkaloids of Tylophora indica (Burm) Merrill and Tylophora dalzellii f. Journal of Pharmaceutical Science, 60(11): 1725-6.

[4] Mulchandani, N. B., Iyer, S. S., and Badheka, L. P. 1971. Structure of tylophorinidine. A new potential antitumor alkaloid from Tylophora indica. Chem India, 19: 505-506.

[5] Sharma, N. and Chandel, K. P. S. 1992. Effects of ascorbic acid on axillary shoot induction in Tylophora indica (Burm. F) Merrill. Plant Cell Tissue Organ Culture, 29: 109-113.

[6] Manjula, S., Job, A. and Nair, G. M. 2000. Somatic embryogenesis from leaf derived callus of Tylophora indica (Burm. f.) Merrill. Indian Journal of Experimental Biology, 38(10): 1069-1072.

[7] Mohammad Faisal. and Mohammad Anis. 2003. Rapid mass propagation of Tylophora indica Merrill via leaf callus culture. Plant Cell Tissue Organ Culture, 75(2): 125-129

[8] Chaudhuri, K. N., Ghosh, , and Jha, S. 2004. The root: a potential new source of competent cells for high-frequency regeneration in Tylophora indica. Plant Cell Reports 22(10): 73-740.

[9] Murashige, T. and Skoog, F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15: 473-497.

[10] Yong-Eui Choi., Deok-Chun Yang., and Eui-Soo Yoon. 1999. Rapid propagation of Eleutherococcus senticosus via direct somatic embryogenesis from explants of seedlings. Plant Cell Tissue Organ Culture, 58: 93-97.

[11] Vendrame, W. A., Holliday, C. P., and Merkle, S. A. 2001. Clonal propagation of hybrid sweetgum (Liquidambar styraciflua x Foemosana) by somatic embryogenesis. Plant Cell Reports, 20: 691-695.

[12] Parra, R. and Amo-Marco, J. B. 1998. Secondary somatic embryogenesis and plant regeneration in myrtle (Myrtus communis). Plant Cell Reports, 18:325-330.

[13] Salunkhe, C. K., Rao, P. S., and Minal Matre. 1997. Induction of somatic embryogenesis and plantlets in tendril of Vitis vinifera Plant Cell Reports, 17: 65-67.

[14]Iantcheva, A., Vlahova, M., Bakalova, E., Kondosori, E., Elliott, M. C., and Atanassov, A. Regeneration of diploid annual medics via direct somatic embryogenesis promoted by thidiazuron and benzylaminopurine. Plant Cell Reports, 18: 904-910.

[15] Ravishankar Rai, V. and Jen McComb. Direct somatic embryogenesis from mature embryos of sandalwood. Plant Cell Tissue Organ Culture, 69: 65-70.

[16] Ammirato, P. V. 1977. Hormonal control of somatic embryo development from cultured cells of carrway. Plant Physiology, 34: 630-637.

[17] Vasil, V. and Vasil, K. 1982. Characterization of an embryonic cell suspension culture derived from cultured inflorescence of Pennesetum americanum (Pearl millet). American Journal of Botany, 699: 1441-1449.

[18] Pattanik, J., Sahoo, S., and Debata, B. K. 1997. Somatic embryogenesis and plantlet regeneration from cell suspension culture of palmorosa grass (Cymbopogon martini). Plant Cell Reports, 16: 430-434.

[19] Chengalaryan, K., Mhaske, V. B., and Hazra, S. 1997. High frequency conversion of abnormal peanut somatic embryos. Plant Cell Reports, 16: 783-786.

[20] A. Rashid. 2002. Induction of multiple shoots by thidiazuron from caryopsis cultures of minor millet (Paspalum scrobiculatum L.) and its effect on the regeneration of embryogenic callus cultures. Plant Cell Reports, 21: 9-13.

[21] Gray, D. J., McColley, D. W., and Compton, M. E. 1992. Effects of cytokinins, genotype and other factors on somatic embryogenesis from cotyledons of Cucumis melo. In vitro Cell Developmental Biology Plant, 28: 101A.

[22] Compton, M. E. and Gray, D. J. 1992. Somatic embryogenesis on immature cotyledons on watermelon. In vitro Cell Developmental Biology Plant, 28: 98A.

[23] Qureshi, J. A. and Saxena, P. K. 1992. Adventitious shoot induction and somatic embryogenesis with intact seedlings of several hybrid seed geranium (Pelargonium x hortorum Bailey) varieties. Plant Cell Reports, 11: 443-448.

[24] Lu, C. Y. 1993. The use of thidiazuron in tissue culture. In vitro Cell Developmental Biology Plant, 29: 92-96.

[25] Redenbaugh, K., Nichol, J., Kossler, M. E., and Paasch, B. 1984. Encapsulation of somatic embryos for artificial seed production. In vitro Cell Developmental Biology Plant, 20: 256-257.

[26] Bapat, V. A. and Rao, P. S. 1988. Somatic embryogenesis and plantlet formation in tissue cultures of Sandal Wood (Santalum album ). Annals of Botany, 44: 629.

[27] Gupta, P. K. and Durzan, D. J. 1987. Biotechnology of somatic polyembroyonesis and plantlet regeneration in Lobolly pine. Biotechnology, 15: 147-151.


ARTICLE INFORMATION



Accepted: 2005-11-30
Available Online: 2006-04-04


Cite this article:

Chandrasekhar, T., Mohammad Hussain, T., Rama Gopal, G., Srinivasa Rao, J.-V. 2006. Somatic embryogenesis of Tylophora indica (Burm.f.) Merril., an important medicinal plant. International Journal of Applied Science and Engineering. 4, 33–40. https://doi.org/10.6703/IJASE.2006.4(1).33

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