Bing-Lan Liu1, Tzyy-Min Rou1, Yerra Koteswara Rao2, Yew-Min Tzeng2*

1 Department of Applied Chemistry,
2 Institute of Biotechnology, Chaoyang University of Technology,Wufong, Taichung County 41349, Taiwan, R.O.C.

 

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ABSTRACT

The effects of culture medium initial pH ranging from pH 2 to 9 on the production ofdestruxins A (DA) and B (DB) from the fungus of Metarhizium anisopliae F061 were examinedby shaker flask culture, in 5 L stirred tank bioreactor (STR) and 20 L airlift bioreactor (ALR). Inshaker flasks, when no control was applied, whatever initial pH used in the medium, the final pHof the broth was always dropped between 3 and 4 at the end of cultivation. During this study,when initial pH was held at below 5, low concentration of DB was obtained. However, the initialpH at 9 is more favorable for the maximal DB production; in contrast, less distinction of DAconcentration was observed within the pH range studied. On the other hand, the pH valuesshowed different effects on DA and DB production in 5 L STR cultivation. Maximum yield ofDA (71.0 mg/L) and DB (310.6 mg/L) were achieved at pH 9 after 12 and 14 day cultivations,respectively, and these values were three fold higher than those obtained from pH of 2 cultivation.Furthermore, culture medium final pH at 3.7 is more favorable for the high yield of DBproduction in the above studies. In 20 L ALR with uncontrolled pH, the maximum levels 350 and550 mg/L of DB were obtained, after 12 and 9 day cultivations by using 6 mesh and without(bubble column mode), net-draft-tube, respectively. On the other hand, the concentration of DBwas greatly increased by increasing the aeration rate from 0.5 to 1.5 vvm (volume per volumeper minute). A maximum yield, nearly 700.0 mg/L of DB was obtained with an aeration rate of1.5 vvm and controlled pH at 6 in bubble column mode cultivation.


Keywords: Metarhizium anisopliae; Destruxins; Airlift bioreactor


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ARTICLE INFORMATION



Accepted: 2007-05-28
Available Online: 2007-07-05


Cite this article:

Liu, B.-L., Rou, T.-M., Rao, Y.-K., Tzeng, Y.-M. 2007. Effect of pH and aeration rate on the production of destruxins A and B from Metarhizium anisopliae. International Journal of Applied Science and Engineering, 5, 17–26. https://doi.org/10.6703/IJASE.2007.5(1).17

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