International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Hung-Yuh Lina, Yerra Koteswara Raob, Wen-Shi Wuc and Yew-Min Tzengb1

a Institute of Biotechnology, National Dong Hwa University, Hualien, Taiwan, R.O.C.
b Institute of Biochemical Sciences and Technology, Chaoyang University of Technology, Wufeng, Taiwan, R.O.C.
c Department of Horticulture and Biotechmology, Chinese Culture University, Taipei, Taiwan, R.O.C.

 

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ABSTRACT

The effect of different carbon sources, initial pH, and addition of divalent metal cations to the shaker flask cultivation medium was observed with the aim of improving iturin A production from Bacillus amyloliquefaciens B128. Among different C-sources tested, soft sugar enhanced iturin A production to 68.78 mg/L, which is 5 fold higher than nutrient broth cultivation concentration. This concentration further enhanced to 71.34 mg/L, after 6 days cultivation by the application of starting cultivation pH at 6.64. Furthermore, when this medium was supplemented with 0.2 mM of iron, iturin A concentration increased to 92.78 mg/L, after 5 days with no pH control cultivation. Interestingly, with the stating pH at 6.64 and 0.2 mM of ferrous sulfate, the highest iturin A production of 121.28 mg/L was obtained. Thus, the present study provides useful information for enhancing iturin A production in cultivation.


Keywords: Bacillus amyloliquefaciens B128; Carbon source; Divalent metal addition; Iturin A.


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ARTICLE INFORMATION



Accepted: 2007-11-20
Available Online: 2007-12-01


Cite this article:

Lin, H.-Y., Rao, Y.K., Wu, W.-S., Tzeng, Y.-M. 2007. Ferrous ion enhanced lipopeptide antibiotic iturin a production from Bacillus amyloliquefaciens B128. International Journal of Applied Science and Engineering, 5, 123–132. https://doi.org/10.6703/IJASE.2007.5(2).123

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