International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Yu-Fong Siea, Hui-Chun Yangb, and Yen Leea1

a National Taitung University, Life science Institute. 684 Sec.1, Chung Hua Rd.Taitung, Taiwan. 950
b Taitung Hospital, Department of Health, Executive Yuan. 1, Wu Chuan St. Taitung, Taiwan. 950

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ABSTRACT

An aerobic, Gram negative extracellular agarase producing bacillus was discovered in Taitung, Taiwan. It has a small plasmid containing an agarase gene with high enzyme activity. This bacterium was identified as Alcaligenes sp. strain Yen (AY744384) by 16S RNA sequencing comparison and MicroScan-AS4 automatic microorganism analyzing data. The intact plasmid was successfully transformed into competent E. coli HB 101 cells for the first time. The enzyme was purified from culture supernatant. The enzyme had a specific agarase activity of 406 U/mg. The plasmid was estimated 18.4 kb by comparing to electrophoresis markers. This agarase couldn’t degrade ι-carrageenan and κ-carrageenan, but could degrade sodium alginate. The enzyme’s acting target was β-1,4 bond, so, the enzyme was recognized as β type. This is a discovery of a previously undescribed small agarolytic plasmid and the first time transformation of the intact agarase gene containing plasmid into E.coli. Some enzyme characteristics were also described.


Keywords: agarolytic bacterium; agarase plasmid; Alcaligenes sp. strain Yen


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ARTICLE INFORMATION



Accepted: 2009-03-12
Available Online: 2009-10-01


Cite this article:

Sie, Y.-F., Yang, H.-C., Lee, Y. 2009. The discoveery of agarolytic bacterium with agrarse gene containing plasmid, and sone enzymology characteristics. International Journal of Applied Science and Engineering, 7, 25–41. https://doi.org/10.6703/IJASE.2009.7(1).25

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