International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Kilambi Pundarikakshudu1 and Hiral N. Dave

L. J. Institute of Pharmacy, Nr. Nagdev kalyan Mandir, Sanand Cross road, Ahmedabad, PIN Code – 382 210 Gujarat, India

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A simple, rapid, accurate, precise, reliable and economical first derivative spectrophotometric method has been proposed for the simultaneous determination of curcumin (CUR) and berberine (BER) in pure form and in combined methanolic extracts of Curcuma longa and Berberis aristata without any prior separation or purification step. The developed methods show best results in terms of linearity, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ) for standard laboratory mixtures of pure compounds and methanolic extracts of the drugs. The linearity range for curcumin (CUR) and berberine (BER) were found to be 2 to 8 µg mL-1and 6 to 12 µg mL-1 respectively. The values of LOD were 0.039 µg mL-1 and 0.025 µg mL-1 and the values of LOQ were found to be 0.13 µg mL-1 and 0.08345 µg mL-1 for curcumin (CUR) and berberine (BER) respectively. By this method, C. longa and B. aristata showed 4% w/w curcumin and 2.8% w/w berberine respectively in combined methanolic extracts of the two herbal drugs. The method was validated as per ICH guidelines.

Keywords: simultaneous determination; derivative spectroscopy; curcumin; berberine; Curcuma longa; Berberis aristata

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Accepted: 2010-09-07
Available Online: 2010-10-01

Cite this article:

Pundarikakshudu, K., Dave, H.N. 2010. Simultaneous determination of curcumin and berberine in their pure form and from the combined extracts of Curcuma Longa and Berberis Aristata. International Journal of Applied Science and Engineering, 8, 19–26.

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