International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Gottumukkala V. Subbarajua*, Sukala Kannababua, Katta Vijayakumara, Papolu B. S. Murthya, Mulabagal Vanisreeb, and Hsin-Sheng Tsayb

a Laila Impex Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada –520 007, Andhra Pradesh, India
b Institute of Biotechnology, Chaoyang University of Technology, Wufong, Taichung 413, Taiwan


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A simple, sensitive and precise spectrophotometric method has been developed for the determination of L-5-Hydroxytryptophan in Griffonia simplicifolia seed extracts and dosage forms. L-5-Hydroxytryptophan gives a blue colored chromogen with Folin-Ciocalteu reagent under alkaline conditions with absorption maxima at 736 nm. The chromogen obeys Beer’s law in the concentration range 1-10 mg/ml. The proposed method is reproducible and statistically validated and recoveries range between 98.95% and 99.96%.

Keywords: Spectrophotometry; L-5-Hydroxytryptophan; Folin-Ciocalteu reagent; Griffonia simplicifolia.

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Accepted: 2005-05-04
Available Online: 2005-10-03

Cite this article:

Subbaraju, G.-V., Kannababu, S., Vijayakumar, K., Murthy,  P.B.S., Vanisree, M., Tsay, H.-S. 2005. Spectrophotometric estimation of L- 5-Hydroxytryptophan in Griffonia simplicifolia extracts and dosage formst. International Journal of Applied Science and Engineering, 3, 111–116.

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