International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Gottumukkala V. Subbarajua*, Sukala Kannababua, Katta Vijayakumara, Papolu B. S. Murthya, Mulabagal Vanisreeb, and Hsin-Sheng Tsayb

a Laila Impex Research Centre, Unit I, Phase III, Jawahar Autonagar, Vijayawada –520 007, Andhra Pradesh, India
b Institute of Biotechnology, Chaoyang University of Technology, Wufong, Taichung 413, Taiwan


 

Download Citation: |
Download PDF


ABSTRACT


A simple, sensitive and precise spectrophotometric method has been developed for the determination of L-5-Hydroxytryptophan in Griffonia simplicifolia seed extracts and dosage forms. L-5-Hydroxytryptophan gives a blue colored chromogen with Folin-Ciocalteu reagent under alkaline conditions with absorption maxima at 736 nm. The chromogen obeys Beer’s law in the concentration range 1-10 mg/ml. The proposed method is reproducible and statistically validated and recoveries range between 98.95% and 99.96%.


Keywords: Spectrophotometry; L-5-Hydroxytryptophan; Folin-Ciocalteu reagent; Griffonia simplicifolia.


Share this article with your colleagues

 


REFERENCES


  1. [1] Murray, N. D. M. 1998. “5-HTP - The natural way to overcome Depression, Obesity and Insomnia”. Bantom Books, New York.

  2. [2] Birdsall, T. C. 1998. 5-Hydroxytryptophan: Clinically effective serotonin precursor. Alternative Medicine Review, 3: 271-280.

  3. [3] Tachiki, K. H. and Aprison, M. H. 1975. Fluorometric assay for 5-hydroxytryptophan with sensitivity in the picomole range. Analytical Chemistry, 47: 7-11.

  4. [4] Petritis, K., Valleix, A., Elfakir, C., and Dreux, M. 2001. Simultaneous analysis of underivatized chiral amino acids by liquid chromatography – ion spray tandem mass spectrometry using teicoplanins chiral stationary phase. Journal of Chromatography A, 913: 313-340.|

  5. [5] Catlabeni, F., Koslow, S. H., and Costa, E. 1972. Gas Chromatographic - mass spectrometric assay of four indole alkylamines of rat pineal. Science, 178: 162-166.

  6. [6] Meek, J. L. 1976. Application of inexpensive equipment for high pressure liquid chromatography to assays for taurine, g-aminobutyric acid and 5-hydroxytryptophan. Analytical Chemistry, 48: 375-379.

  7. [7] Mefford, N. and Burchard, J. D. 1980. Determination of metabolites in rat brains and pineal tissue by reversed phase high-performance liquid chromatography with electro chemical detection. Journal of Chromatography, 181: 187-193.

  8. [8] Arneric, S. P., Goodale, D. B., Flynn, J.R., and Long, J.P. 1981. Rapid and simple analysis of Dopa and 5-HTP using high performance liquid chromatography with electrochemical detection, Brain Research Bulletin, 6: 407-411.

  9. [9] Calguri, E. J. and Mefford, N. 1984. Femtogram detection limits for biogenic amines using microbore HPLC with electro chemical detection. Brain Research, 296: 156-159.

  10. [10] Yi, Y. H., Liao, W. P., and Lu, X. 1994. Simultaneous determination of tryptophan, 5-hydroxytryptophan, 5-hydroxytryptamine, 5-hydroxyindoleacetic acid, 5- hydroxyphenylglycol in human cerebrospinal fluid, Journal of Chromatography B, 661: 143-148.

  11. [11] Lemaire, P. A. and Adosraku, R. K. 2002. An HPLC method for the direct assay of the serotonin precursor, 5-hydroxytryptophan, in seeds of Griffonia simplicifolia. Phytochemical Analysis, 13: 333-337.

  12. [12] Kannababu, S., Ramakrishna, T., and Subbaraju, G.V. 2005. HPLC estimation of 5-hydroxytryptophan in Griffonia simplicifolia Asian Journal of Chemistry, 17: 506- 510.

  13. [13] Takagai, Y. and Igarashi, S. 2003. Determination of ppb levels of tryptophan derivatives by capillary electrophoresis with homogeneous liquid-liquid extraction and sweeping method. Chemical and Pharmaceutical Bulletin, 51: 373-377.

  14. [14] Massart, D.L., Vandeginste, B.G.M., Deming, S.N., Michotte, Y., and Kaufman, L. 1988. “Chemometrics: A Text Book”. Elsevier, Amsterdam.

  15. [15] Devala Rao, G. 2004. Development and Validation of spectrophotometric method for the determination of anti-HIV drug. Asian Journal of Chemistry, 16: 1217-1219.


ARTICLE INFORMATION




Accepted: 2005-05-04
Available Online: 2005-10-03


Cite this article:

Subbaraju, G.-V., Kannababu, S., Vijayakumar, K., Murthy,  P.B.S., Vanisree, M., Tsay, H.-S. 2005. Spectrophotometric estimation of L- 5-Hydroxytryptophan in Griffonia simplicifolia extracts and dosage formst. International Journal of Applied Science and Engineering, 3, 111–116. https://doi.org/10.6703/IJASE.2005.3(2).111


We use cookies on this website to improve your user experience. By using this site you agree to its use of cookies.