International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Wen-Kai Lua, Tzu-Yu Chiub, Shu-Hsien Hunga,b, Ing-Lung Shihc, Yaw-Nan Changa,b*

Department of Food Engineering
Department of Bioindustry Technology
Department of Environmental Engineering, Da-Yeh University,Changhua, 515, Taiwan, R.O.C.


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ABSTRACT


Response surface methodology (RSM) was employed to study the effects of four medium components (L-glutamic acid, citric acid, glycerol and NH4Cl) on the production of poly-γ-glutamic acid (PGA) in flask cultures by Bacillus licheniformis CCRC 12826 (ATCC 9945) with initial medium pH 6.5. The optimal PGA yield (35.34 g/L, average of four repeats) was appeared at the region where the respective concentrations (in g/L) of glutamic acid, citric acid, glycerol and NH4Cl were around 34.0, 26.0, 146.0 and 11.0, respectively. The optimized composition derived from RSM regression was (in g/L) glutamic acid 34.70, citric acid 27.00, glycerol, 145.45 and NH4Cl 10.49. With this composition, the PGA production was 33.20 g/L (average of eight repeats) after 96 h of cultivation, while the predicted maximum production was 35.52 g/L. The optimized medium resulted in significant increase of the PGA yield by B. licheniformis CCRC 12826 in shake-flask cultivation without any feeding process. In comparison with glutamic acid, citric acid and glycerol, NH4Cl represents a crucial role in PGA biosynthesis.


Keywords: Bacillus licheniformis; response surface methodology; poly-γ-glutamic acid


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ARTICLE INFORMATION




Accepted: 2004-02-20
Available Online: 2004-03-02


Cite this article:

Lu, W.-K., Chiu, T.-Y., Hung, S.H., Shih, I.-L., Chang, Y.-N., 2004. Use of response surface methodology to optimize culture medium for production of Poly-γ-glutamic acid by bacillus licheniformis, International Journal of Applied Science and Engineering, 2, 49–58. https://doi.org/10.6703/IJASE.2004.2.(1).49


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