International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Alluri V. Krishnaraju1, Tayi V. N. Rao1, Dodda Sundararaju1, Mulabagal Vanisree2,Hsin-Sheng Tsay2, and Gottumukkala V. Subbaraju1*

Laila Impex Research Centre, Unit I, Phase III, 1Laila Impex Research Centre, Unit I, Phase III,Jawahar Autonagar, Vijayawada –520 007, India.
2 Institute of Biotechnology, Chaoyang University of Technology,Wufong, Taichung 413, Taiwan


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Medicinal plants constitute important components of flora and are widely distributedin different regions of India. Based on ethnomedical significance, we have collected several medicinalplants used in traditional medicine from Eastern Ghats of India and evaluated for theirbiological activity. In the present study, a method utilizing brine shrimp (Artemia salina Leach)lethality was used to screen medicinal plants for their biological activity. Aqueous extracts from118 Indian medicinal plants were screened by the brine shrimp lethality assay and found elevenout of the 118 extracts showed significant toxicity to the brine shrimp (<60 μg/ml). Polygonumcuspidatum and Syzygium cumini extracts have exhibited potent activity with LC50 13.5 and 20,respectively. The results were analyzed within the context of the available traditional knowledgeand uses for these plants. Present study could be useful in the search for new antitumor compoundsfrom the Indian flora.

Keywords: Artemia salina; cytotoxicity, brine shrimp; Indian medicinal plants

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Accepted: 2006-06-15
Available Online: 2006-09-01

Cite this article:

Krishnaraju, A.-V., Rao, T.V.-N. , Sundararaju, D., Vanisree, M., Tsay, H.-S., Subbaraju, G.-V. 2006. Biological screening of medicinal plants collected from eastern ghats of India using artemia salina(Brine Shrimp Test). International Journal of Applied Science and Engineering, 4, 115–125.