International Journal of Applied Science and Engineering
Published by Chaoyang University of Technology

Hemant Shigwanb*, Arvind Saklania, P. D. Hamrapurkarb, Tukaram Manea, and Priyanka Bhattb

aPiramal Life Sciences limited, 1, Nirlon Complex, Off. Western Express Highway,Goregaon (East), Mumbai, Maharashtra, India
bPharmaceutical Analysis, Principal K. M. Kundnani College of Pharmacy, Jote Joy Building, Rambhau Salgaoankar Marg, Cuffe Parade, Mumbai, India


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An RP-HPLC method with photodiode array detection has been developed for the determination of major constituent berberine from Berberis aristata (Barberry) and Berberis tinctoria. Berberine was isolated from the plant extract on semi-preparative HPLC and separated on HPLC by using an isocratic mode consisting of 0.1% trifloroacetic acid: acetonitrile (60:40, v/v) at a flow rate of 1 mL/min. Under these conditions, a plot of integrated peak area versus concentration of berberine was found be linear over the concentration range of 0.2 µg/mL to 150 µg/mL. The limit of detection was 1ng on column and limit of quantification was 2 ng on column for berberine. The berberine content in B. aristata and B. tinctoria was found to be 3.18% and 1.46% respectively.

Keywords: HPLC Method Development and Validation for Quantification of Berberine from Berberis aristata and Berberis tinctoria

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Received: 2012-10-15
Revised: 2013-03-21
Accepted: 2013-03-21
Available Online: 2013-06-01

Cite this article:

Shigwan, H., Saklani, A., Hamrapurkar, P.D., Mane, T., Bhatt, P. 2013. HPLC method development and validation for quantification of berberine from berberis aristata and berberis tinctoria. International Journal of Applied Science and Engineering, 11, 203–211.